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Alkaline hydrogen peroxide pretreatment of bamboo culm for improved enzymatic release of reducing sugars using recombinant cellulases

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Abstract

Bamboo (Phyllostachys acuta) is considered one of the useful feedstocks of crop residues due to speedy growth, fastest propagation, and convenient harvesting. The influence of alkaline hydrogen peroxide (AHP) pretreatment of bamboo culm, its structural changes, and enzymatic hydrolysis were determined. Scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction were used for the analysis of solid fraction after alkaline hydrogen peroxide treatment of bamboo culm. Recombinant enzymes were expressed in Pichia pastoris from newly identified Aspergillus niger BE-2. The obtained results revealed more hemicelluloses hydrolysis and improved cellulose accumulation in degraded part. The cellulose component was increased by 36.87%, hemicellulose decreased by 50.66%, and lignin by 37.94% in comparison with the chemical components in the raw material after AHP pretreatment. There is 111% increased yield reported for recombinant enzymes expressed in Pichia pastoris after 60 h of degradation as compared to untreated substrates biomass. A total of about 370 mg reducing sugars per gram dehydrated bamboo residues were obtained after AHP treatment. The results revealed that major structural changes take place in the physiology of the substrates after AHP treatment, including elimination of lignin and hemicellulose, and enhance the porous area for easy attack of recombinant cellulases. This investigation contributes in biomass conversion in a friendly environment to fulfil the energy requirement in the future challenges.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 21303142 & No. 31170067), the research fund from the Oceans and Fisheries bureau of Xiamen (No. 14GZP59HJ29), and Fujian Provincial Department of Ocean and Fisheries (No. 2014-25). We are also grateful to CAS President’s International Fellowship Initiative Program and School of Environment, Tsinghua University for additional financial support.

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Authors and Affiliations

  1. School of Life Science, Xiamen University, Xiamen, 361005, People’s Republic of China

    Nasir Ali

  2. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, 100084, People’s Republic of China

    Nasir Ali & Xiang Liu

  3. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, Shandong Province, People’s Republic of China

    Nasir Ali & Mohnad Abdalla

  4. Green Intelligence Environmental School, Yangtze Normal University, Chongqing, 408003, People’s Republic of China

    Abdulmoseen Segun Giwa

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  1. Nasir Ali
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  4. Xiang Liu
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Correspondence to Nasir Ali.

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Ali, N., Giwa, A.S., Abdalla, M. et al. Alkaline hydrogen peroxide pretreatment of bamboo culm for improved enzymatic release of reducing sugars using recombinant cellulases. Cellulose 27, 769–779 (2020). https://doi.org/10.1007/s10570-019-02829-8

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